Phosphocreatine Interacts with Phospholipids, Affects Membrane Properties and Exerts Membrane-Protective Effects
Identifieur interne : 000118 ( France/Analysis ); précédent : 000117; suivant : 000119Phosphocreatine Interacts with Phospholipids, Affects Membrane Properties and Exerts Membrane-Protective Effects
Auteurs : Malgorzata Tokarska-Schlattner [France] ; Raquel F. Epand [Canada] ; Flurina Meiler [Suisse] ; Giorgia Zandomeneghi [Suisse] ; Dietbert Neumann [Suisse] ; Hans R. Widmer [Suisse] ; Beat H. Meier [Suisse] ; Richard M. Epand [Canada] ; Valdur Saks [France] ; Theo Wallimann [Suisse] ; Uwe Schlattner [France]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Abstract
A broad spectrum of beneficial effects has been ascribed to creatine (Cr), phosphocreatine (PCr) and their cyclic analogues cyclo-(cCr) and phospho-cyclocreatine (PcCr). Cr is widely used as nutritional supplement in sports and increasingly also as adjuvant treatment for pathologies such as myopathies and a plethora of neurodegenerative diseases. Additionally, Cr and its cyclic analogues have been proposed for anti-cancer treatment. The mechanisms involved in these pleiotropic effects are still controversial and far from being understood. The reversible conversion of Cr and ATP into PCr and ADP by creatine kinase, generating highly diffusible PCr energy reserves, is certainly an important element. However, some protective effects of Cr and analogues cannot be satisfactorily explained solely by effects on the cellular energy state. Here we used mainly liposome model systems to provide evidence for interaction of PCr and PcCr with different zwitterionic phospholipids by applying four independent, complementary biochemical and biophysical assays: (i) chemical binding assay, (ii) surface plasmon resonance spectroscopy (SPR), (iii) solid-state 31P-NMR, and (iv) differential scanning calorimetry (DSC). SPR revealed low affinity PCr/phospholipid interaction that additionally induced changes in liposome shape as indicated by NMR and SPR. Additionally, DSC revealed evidence for membrane packing effects by PCr, as seen by altered lipid phase transition. Finally, PCr efficiently protected against membrane permeabilization in two different model systems: liposome-permeabilization by the membrane-active peptide melittin, and erythrocyte hemolysis by the oxidative drug doxorubicin, hypoosmotic stress or the mild detergent saponin. These findings suggest a new molecular basis for non-energy related functions of PCr and its cyclic analogue. PCr/phospholipid interaction and alteration of membrane structure may not only protect cellular membranes against various insults, but could have more general implications for many physiological membrane-related functions that are relevant for health and disease.
Url:
DOI: 10.1371/journal.pone.0043178
PubMed: 22912820
PubMed Central: 3422282
Affiliations:
- Canada, France, Suisse
- Auvergne-Rhône-Alpes, Canton de Berne, Canton de Zurich, Ontario, Rhône-Alpes
- Berne, Grenoble, Hamilton (Ontario), Zurich
- Université McMaster
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PMC:3422282Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>A broad spectrum of beneficial effects has been ascribed to creatine (Cr), phosphocreatine (PCr) and their cyclic analogues cyclo-(cCr) and phospho-cyclocreatine (PcCr). Cr is widely used as nutritional supplement in sports and increasingly also as adjuvant treatment for pathologies such as myopathies and a plethora of neurodegenerative diseases. Additionally, Cr and its cyclic analogues have been proposed for anti-cancer treatment. The mechanisms involved in these pleiotropic effects are still controversial and far from being understood. The reversible conversion of Cr and ATP into PCr and ADP by creatine kinase, generating highly diffusible PCr energy reserves, is certainly an important element. However, some protective effects of Cr and analogues cannot be satisfactorily explained solely by effects on the cellular energy state. Here we used mainly liposome model systems to provide evidence for interaction of PCr and PcCr with different zwitterionic phospholipids by applying four independent, complementary biochemical and biophysical assays: (i) chemical binding assay, (ii) surface plasmon resonance spectroscopy (SPR), (iii) solid-state <sup>31</sup>
P-NMR, and (iv) differential scanning calorimetry (DSC). SPR revealed low affinity PCr/phospholipid interaction that additionally induced changes in liposome shape as indicated by NMR and SPR. Additionally, DSC revealed evidence for membrane packing effects by PCr, as seen by altered lipid phase transition. Finally, PCr efficiently protected against membrane permeabilization in two different model systems: liposome-permeabilization by the membrane-active peptide melittin, and erythrocyte hemolysis by the oxidative drug doxorubicin, hypoosmotic stress or the mild detergent saponin. These findings suggest a new molecular basis for non-energy related functions of PCr and its cyclic analogue. PCr/phospholipid interaction and alteration of membrane structure may not only protect cellular membranes against various insults, but could have more general implications for many physiological membrane-related functions that are relevant for health and disease.</p>
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</analytic>
</biblStruct>
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</analytic>
</biblStruct>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, Sk" uniqKey="Lee S">SK Lee</name>
</author>
<author><name sortKey="Lee, Bm" uniqKey="Lee B">BM Lee</name>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tokarska Schlattner, M" uniqKey="Tokarska Schlattner M">M Tokarska-Schlattner</name>
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</biblStruct>
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</biblStruct>
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<author><name sortKey="Coyle, Jt" uniqKey="Coyle J">JT Coyle</name>
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</analytic>
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</author>
<author><name sortKey="Lauderback, Cm" uniqKey="Lauderback C">CM Lauderback</name>
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</biblStruct>
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</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rodriguez, M" uniqKey="Rodriguez M">M Rodriguez</name>
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</analytic>
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</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>Canada</li>
<li>France</li>
<li>Suisse</li>
</country>
<region><li>Auvergne-Rhône-Alpes</li>
<li>Canton de Berne</li>
<li>Canton de Zurich</li>
<li>Ontario</li>
<li>Rhône-Alpes</li>
</region>
<settlement><li>Berne</li>
<li>Grenoble</li>
<li>Hamilton (Ontario)</li>
<li>Zurich</li>
</settlement>
<orgName><li>Université McMaster</li>
</orgName>
</list>
<tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Tokarska Schlattner, Malgorzata" sort="Tokarska Schlattner, Malgorzata" uniqKey="Tokarska Schlattner M" first="Malgorzata" last="Tokarska-Schlattner">Malgorzata Tokarska-Schlattner</name>
</region>
<name sortKey="Saks, Valdur" sort="Saks, Valdur" uniqKey="Saks V" first="Valdur" last="Saks">Valdur Saks</name>
<name sortKey="Saks, Valdur" sort="Saks, Valdur" uniqKey="Saks V" first="Valdur" last="Saks">Valdur Saks</name>
<name sortKey="Schlattner, Uwe" sort="Schlattner, Uwe" uniqKey="Schlattner U" first="Uwe" last="Schlattner">Uwe Schlattner</name>
<name sortKey="Schlattner, Uwe" sort="Schlattner, Uwe" uniqKey="Schlattner U" first="Uwe" last="Schlattner">Uwe Schlattner</name>
<name sortKey="Tokarska Schlattner, Malgorzata" sort="Tokarska Schlattner, Malgorzata" uniqKey="Tokarska Schlattner M" first="Malgorzata" last="Tokarska-Schlattner">Malgorzata Tokarska-Schlattner</name>
</country>
<country name="Canada"><region name="Ontario"><name sortKey="Epand, Raquel F" sort="Epand, Raquel F" uniqKey="Epand R" first="Raquel F." last="Epand">Raquel F. Epand</name>
</region>
<name sortKey="Epand, Richard M" sort="Epand, Richard M" uniqKey="Epand R" first="Richard M." last="Epand">Richard M. Epand</name>
</country>
<country name="Suisse"><region name="Canton de Zurich"><name sortKey="Meiler, Flurina" sort="Meiler, Flurina" uniqKey="Meiler F" first="Flurina" last="Meiler">Flurina Meiler</name>
</region>
<name sortKey="Meier, Beat H" sort="Meier, Beat H" uniqKey="Meier B" first="Beat H." last="Meier">Beat H. Meier</name>
<name sortKey="Neumann, Dietbert" sort="Neumann, Dietbert" uniqKey="Neumann D" first="Dietbert" last="Neumann">Dietbert Neumann</name>
<name sortKey="Wallimann, Theo" sort="Wallimann, Theo" uniqKey="Wallimann T" first="Theo" last="Wallimann">Theo Wallimann</name>
<name sortKey="Widmer, Hans R" sort="Widmer, Hans R" uniqKey="Widmer H" first="Hans R." last="Widmer">Hans R. Widmer</name>
<name sortKey="Zandomeneghi, Giorgia" sort="Zandomeneghi, Giorgia" uniqKey="Zandomeneghi G" first="Giorgia" last="Zandomeneghi">Giorgia Zandomeneghi</name>
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</tree>
</affiliations>
</record>
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